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. 2014 Dec 18;15:27. doi: 10.1186/s12858-014-0027-0

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© Nayak et al.; licensee BioMed Central. 2014

This is an Open Access article distributed under the terms of the Creative Commons Attribution License (http://creativecommons.org/licenses/by/4.0), which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly credited. The Creative Commons Public Domain Dedication waiver (http://creativecommons.org/publicdomain/zero/1.0/) applies to the data made available in this article, unless otherwise stated.

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GST-DUSP5 and GST-S147P proteins are active against pERK in vitro. Panels (A-B) are p-ERK western blots performed with purified WT DUSP5 or S147P proteins. (A) Time course experiment with 5 min and 15 min incubation of DUSP5 recombinant proteins with pERK. (B) Dose curve of recombinant DUSP5 proteins with a 15 min incubation period with pERK.