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. 2014 Dec 29;112(2):560–565. doi: 10.1073/pnas.1414569112

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Fig. 1. — Real-time measurement of roGFP2 in S. Typhimurium. (A and B) Dose-dependent response of intrabacterial redox potential after a challenge with H₂O₂ (A) or SpermineNONOate (B), respectively. The upward arrow indicates injection of the oxidative compound. These experiments are done in LPM at pH 5.8. (C) Correlation between the intrabacterial H₂O₂ concentration and the normalized 405/480 ratio in HpxF(−) Salmonella. (D and E) Microscopic analysis of real-time response to challenge with 2.5 mM H₂O₂ in PBS. The false-colored ratio images are obtained after analysis by ImageJ. The numbers in the images in E correspond to the time points indicated in D.