Fig. 8.

Using intensity measurements can improve the estimate for N. (A) When $N=5$ we can observe, in principle, a fluorescence intensity ranging from one to five bright (if all PA-FPs are on simultaneously). Here we show how many states coincide with the different fluorescence intensities up to three simultaneously bright. (B) We show that we can take advantage of the intensity measurements to compute a correctly centered distribution for N (using 200 synthetic traces with 200 bootstrap iterations).