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. 2014 Dec 29;112(2):E176–E185. doi: 10.1073/pnas.1412984112

Fig. 1.

Fig. 1.

ONNV replication is restricted to midgut cells in An. gambiae 3 d after an infective blood meal. Labeled ONN-EGFP virus was fed to mosquitoes in a blood meal, and tissues were dissected and examined for EGFP fluorescence to detect cellular sites of viral replication. Anterior midgut (A) and posterior midgut (B) 1 d PBM are shown (Scale bar: 50 μm.) Anterior midgut (C) and posterior midgut (D) 3 d PBM are shown. (Scale bar: 50 μm. B′ and D′ enlarge boxed regions.) (E) Salivary glands 7 d PBM. (Scale bar: 20 μm.) (F and G) Circulating hemocyte cells perfused from mosquitoes 7 d PBM. (Scale bar: 20 μm.) (H) RT-PCR detection of viral RNA extracted from mosquito legs shows an absence of detectable virus in systemic circulation 3 d PBM and positive detection of circulating virus 7 d PBM. eGFP, RT-PCR detection of EGFP target in ONN-EGFP viral genome; S7, transcripts of mosquito ribosomal protein S7 as control.