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. 2014 Dec 29;112(2):512–517. doi: 10.1073/pnas.1413291112

Fig. 1.

Fig. 1.

Transduced bone marrow progenitor populations efficiently reconstitute myeloid and lymphoid compartments and develop normally into functional CD8+ T cells. (A) Schematic diagram of transduction strategy. (B) Fraction of LSK cells (Left) transduced with GFP-expressing lentivirus at the time of transplant, and in lymphoid (Middle) and myeloid (Right) cell populations following engraftment. (C) Quantitation of fractions of transduced (GFP+) and untransduced (GFP–), donor-derived T cells following engraftment as in B. (D) Fraction of transduced (GFP+) and untransduced (GFP–), donor-derived naive P14 CD8+ T cells (Left) before adoptive transfer, and of effector (Right) P14 CD8+ T cells in tissues indicated 10 d following transfer and subsequent host infection with PR8-GP33 influenza virus. Representative data are shown from two to three experiments with four to five mice per group.