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. 2014 Dec 29;112(2):418–423. doi: 10.1073/pnas.1423026112

Fig. 3.

Fig. 3.

Dynamics of mLnp1. (A) A diagram showing how mLnp1 dynamics were determined. The average diameter of mLnp1 puncta (green) was approximately 0.75 μm, and a 1.5-μm-diameter circle was used to mark the restricted region. Stationary mLnp1 puncta are defined as those puncta that do not move out of the circle during 180 s. Mobile mLnp1 puncta are defined as those puncta that move entirely out of the circle. (B) The dynamics of mLnp1 puncta were monitored by using mLnp1-GFP. The ER tubules were marked by mCherry-KDEL. The boxed area (Top) is enlarged below. Solid circles (diameter, 1.5 μm) mark the original position of stationary mLnp1 puncta. Dashed circles (diameter, 1.5 μm) mark the original position of mobile mLnp1 puncta. (Scale bars: Top, 4 μm; Bottom, 2 μm.) (C–F) The dynamic properties of mLnp1 puncta are shown through time-lapse imaging. (C) An mLnp1 punctum moves from one junction to another junction along an ER tubule. (D) An mLnp1 punctum merges with another mLnp1 puncta. (E) An mLnp1 punctum splits into two puncta. (F) An mLnp1 punctum moves with a junction as it undergoes sliding. (Scale bars: 2 μm.) Arrowheads in C–F point to mobile mLnp1 puncta. (G) Quantification of mLnp1 dynamics (n = 1,022 mLnp1 puncta).