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. 2014 Dec 29;112(2):418–423. doi: 10.1073/pnas.1423026112

Fig. 4.

Fig. 4.

Preexisting mLnp1-free junctions undergo more movements leading to ring closure. (A–E) The various types of ring closure events that we observed are shown through time-lapse imaging. The ER tubules are marked by mCherry-KDEL. mLnp1 puncta were monitored by using mLnp1-GFP. (A) An mLnp1-free junction moves to another mLnp1-free junction. (B) An mLnp1-free junction moves to a stationary mLnp1 junction. (C) A junction leaves a stationary mLnp1 punctum and moves to another junction. (D) An mLnp1 junction and an mLnp1-free junction move toward each other. (E) An mLnp1 junction moves to a stationary junction. The arrowhead points to a moving junction. The circle marks the original position of an mLnp1 punctum. (Scale bars: 2 μm.) (F) Quantification of the various types of ring closure events (n = 248 ring closures).