Skip to main content
. 2014 Dec 15;141(24):4690–4696. doi: 10.1242/dev.112169

Fig. 3.

Fig. 3.

The transcription factor Isl1 is expressed during the middle stages of Merkel cell differentiation. (A) IF analysis showing the onset of Isl1 expression in Atoh1-GFP+ Merkel cells at E15 and full expression at E16. (B) WMIF shows overlap of Atoh1-GFP and Isl1 in P0 back skin touch-domes. (C) Further analysis shows that Isl1 expression overlaps with Krt20 at P0. (D,E) Analysis of WT and Atoh1cKO back skin at E16 (D) and P0 (E) reveals a complete absence of Isl1 in the Atoh1cKO epidermis. (F-H) IF studies of P0 WT and Sox2cKO epidermis show a partial reduction in both Isl1+ cells (G, P=0.0026) and the intensity of Isl1 fluorescent signal (H, P=0.0014) in Sox2cKO mice. Scale bars: 25 µm.