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. 2014 Dec 15;141(24):4690–4696. doi: 10.1242/dev.112169

Fig. 4.

Fig. 4.

Isl1 is not required for Merkel cell development. (A) IF analysis of P0 WT and Isl1cKO epidermis confirms the loss of Isl1 from K+ (Krt8+) Merkel cells. (B) WMIF reveals no difference in the number of K8+/Atoh1-GFP+ cells per touch-dome in Isl1cKO epidermis (P=0.9283). (C) Sox2 expression is not lost from K8+ Merkel cells in Isl1cKO epidermis, as confirmed by IF at P0. (D-F) IF analysis of mature Merkel cell markers Krt18 (D, P=0.8993), NF200 (E) and Krt20 (F, P=0.8726) shows that Merkel cells are able to reach maturity in Isl1cKO epidermis. (G) IF analysis of E16 Isl1cKO epidermis confirms the loss of Isl1 from developing Merkel cells. (H,I) Analysis of WT and Isl1cKO mice at E16 (H,I) or E17 (I′), using IF against Merkel cell markers K8 (H, P=0.8545), K18 (Krt18) (I, 0.2245) and K20 (Krt20)(I′, 0.6417), shows that the Merkel cell maturation program is unaffected in Isl1cKO embryos. Scale bars: 25 µm.