Fig. 1.

Ras^V12 and sec15 synthetic lethal interaction. (A-D) Intact third instar larval cephalic regions showing wild-type (WT), sec15, Ras^V12 and Ras^V12, sec15 double mutant eye-antenna disc clones. Wild-type (A) and sec15 (B) clones are comparable in size, whereas Ras^V12 clones (C) overgrow to develop tumors. The sec15 mutation suppresses Ras^V12 tumor growth (D). (E-H) Similarly aged eye discs bearing wild-type, sec15, Ras^V12 and Ras^V12, sec15 double mutant clones. Wild-type (E) and sec15 (F) clones show a comparable sparse pattern, whereas Ras^V12 clones (G) overgrow to form large contiguous tumors. However, Ras^V12, sec15 double mutant clones are sparse and smaller (H). (I-L) Adult eyes showing lethality and contribution of larval clones to the eye field. Wild-type (I) and sec15 (J) clones similarly persist into the adult eye and do not cause animal lethality. Ras^V12 clones (K) are pupal lethal but animal lethality is suppressed in animals bearing Ras^V12, sec15 double mutant clones, and cells of this genotype now contribute to the adult eye (L).