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. 2014 Dec 15;141(24):4729–4739. doi: 10.1242/dev.108092

Fig. 2.

Fig. 2.

RasV12 cells upregulate exocyst proteins. (A-G) RasV12 clones stained with antibodies against exocyst proteins (Sec15, Sec6, Sec8 and Rab11) and co-stained with DAPI (blue) to mark nuclei. Lower panels show Sec15, Sec6, Sec8 and Rab11 channels alone. Sec15 is upregulated in RasV12 cells (A). Similarly, Sec6 (B), Sec8 (C) and Rab11 (D) are upregulated in RasV12 clones but not in neighboring wild-type cells. Green dotted lines denote clone boundaries. (E-G) x/z confocal projection images of RasV12 clones (green) stained with anti-Sec8, anti-Rab11 and anti-Sec15, and with DAPI. Sec8 (E), Rab11 (F) and Sec15 (G) proteins display no obvious localization defects but show increased abundance in RasV12 cells. (H) Lysate from discs bearing wild-type (WT) or RasV12 clones immunoblotted with anti-Sec6, anti-Sec8, anti-Sec15 or anti-Tubulin antibodies (loading control). Sec6, Sec8 and Sec15 protein abundance is increased in RasV12 lysates.