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. 2014 Dec 15;141(24):4806–4816. doi: 10.1242/dev.115535

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© 2014. Published by The Company of Biologists Ltd

This is an Open Access article distributed under the terms of the Creative Commons Attribution License (http://creativecommons.org/licenses/by/3.0), which permits unrestricted use, distribution and reproduction in any medium provided that the original work is properly attributed.

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Fig. 1. — Dll1 and Notch1 mRNA expression in mouse PSM. (A-F) In situ hybridisation of Dll1(i) (A-C) and Notch1(i) (D-F) in E10.5 PSM using intronic (i) RNA probes. (G-I) Fix-and-culture assay comparing the expression pattern of Dll1(i) (G) and Notch1(i) (H) with that of the clock gene Hes7 (I). (J-O) FISH of Dll1 (J-L) and Notch1 (M-O) mRNA in PSM using exonic (e) probes. (P-R) qRT-PCR for Dll1, Notch1, Hes7, β-actin and Gapdh in the caudal halves of individual E10.5 PSM explants following fix and culture. Data show the mean ± s.d. of technical replicates. (P,Q) Individual samples showing the fold change in mRNA concentration between fixed and cultured explants for Dll1, Notch1, Hes7 and β-actin once normalised to Gapdh. (R) Total variance of fold change in expression levels in fix:culture ratios of each gene measured by qRT-PCR across all 16 samples. a.u., arbitrary units. **P<0.001.