Table 1.

Protocols used for papain immobilized on chitin.

Treatment	Immobilization conditions	Pretreatment of support
A	1 g chitin + 1% papaina	—
B	1 g chitin + 1% papain + 0.1% PEI	—
C	1 g chitin + 1% papain + 0.5% PEI	—
D	1 g chitin + 1% papain	2% glutaraldehydeb
E	1 g chitin + 1% papain + 0.1% PEIc	2% glutaraldehyde
F	1 g chitin + 1% papain + 0.5% PEI	2% glutaraldehyde

^a10 mL of 10 g·L⁻¹; ^b10 mL of 2% (v·v⁻¹) glutaraldehyde; ^c0.1 or 0.5% (v·v⁻¹) in papain solution.