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Reverse transcription-nested polymerase chain reaction detection of viral shedding in feces. Hepatitis E virus RNA was detected by reverse transcription-nested polymerase chain reaction (RT-nPCR) in feces from all inoculated gerbils. Lane M: DNA marker; Lanes 1-6 represent experimental time points (in weeks): the second round PCR products of gerbil feces samples on intermittent days; Lane 7: a negative control containing water.
