Table 1.
Gene | Forward primer | Reverse primer | Bp | Accession No. |
Notch1 | AGAGCTTTTCCTGTGTCTGTCC | CGGTACAGTCAGGTGTGTTGTT | 414 | NM_001105721 |
Notch2 | GACTGCCAATACTCGACCTC | TTCAGAAGTGAAGTCTCCAG | 438 | NM_024358.1 |
Notch3 | CCTCTTTCACCTGTACCTGTCC | ACACAGTAGTGGGAGTGGTCCT | 496 | NM_020087 |
Notch4 | CAACTCTGCGAGAACGGTGG | TGGAAGGAGCCCAAGGTGTT | 443 | NM_001002827.1 |
hes1 | GCCAGCTGATATAATGGA | TAGGTCATGGCGTTGATC | 462 | NM_024360.3 |
hey1 | CTACAGCTCCTCTGATAGTG | GAGGCATCGAGTCCTTCAAT | 420 | NM_001191845 |
TGF-β1 | GGTGAAACGGAAGCGCATCG | CTTGAATCTCTGCAGGCGCA | 364 | NM_021578.2 |
E-cadherin | CTCGTGGCTTTGTCAGCA | GACCCAGTCTCGTTTCTG | 432 | NM_031334.1 |
α-SMA | GTGTGAAGAGGAAGACAG | TTGGCCTTAGGGTTCAGC | 348 | NM_031004.2 |
β-catenin | CTGACAGAGTTGCTCCACTC | CAGCCCATCAACTGGATAGT | 342 | NM_053357.2 |
Bmp7 | CAGAGCATCAACCCCAAGTT | GATGAAGTGAACCAGTGTCT | 396 | NM_001191856.1 |
COL1α2 | ACCTCAGGGTGTTCAAGGTG | CGGATTCCAATAGGACCAGA | 222 | NM_053356 |
GAPDH | ACCACAGTCCATGCCATCAC | TCCACCACCCTGTTGCTGTA | 480 | NM_017008.4 |