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. 2015 Jan 20;5:764. doi: 10.3389/fpls.2014.00764

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Copyright © 2015 Zhao, Zhang, Zhang, Wu, Zheng, Tian, Liu and Duan.

This is an open-access article distributed under the terms of the Creative Commons Attribution License (CC BY). The use, distribution or reproduction in other forums is permitted, provided the original author(s) or licensor are credited and that the original publication in this journal is cited, in accordance with accepted academic practice. No use, distribution or reproduction is permitted which does not comply with these terms.

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Transcription and histone acetylation levels of GA biosynthesis genes in HDT701 overexpression lines during seed germination. (A) qRT-PCR analysis of the expression levels of GA biosynthetic genes, GA3ox1, GA3ox2, GA20ox2, and GA20ox3 in HDT701 OX4 and OX7. ACTIN was used as the internal control. (B) ChIP analysis of the histone H4 acetylation levels of the promoters and exons of GA biosynthetic genes in germinating HDT701 OX4 and OX7 seeds. P, promoter; E, exon. UBQ was used as the internal control. At least 30 seedlings of WT, HDT701 OX4, and OX7 were used for analysis. Values are shown as means + SD (t-test: ^*P < 0.05, ^**P < 0.01, difference from WT).