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. 2015 Jan 20;5:801. doi: 10.3389/fpls.2014.00801

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Copyright © 2015 Wu, Gong, Yang, Hu, Tai and Wang.

This is an open-access article distributed under the terms of the Creative Commons Attribution License (CC BY). The use, distribution or reproduction in other forums is permitted, provided the original author(s) or licensor are credited and that the original publication in this journal is cited, in accordance with accepted academic practice. No use, distribution or reproduction is permitted which does not comply with these terms.

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2-DE comparison of embryo protein profiles between maize mutant vp5 and wild type Vp5. (A,B) are representative 2-DE gels of pH 4–7 and pH 7–10, respectively. Differentially accumulated protein spots between vp5 and Vp5 embryos are indicated by arrows. Red arrow, increased abundance in vp5; blue arrow, increased abundance in Vp5. Embryo protein was extracted and resolved in 2-DE with IEF in the first dimension and 13.5% SDS-PAGE gel in the second dimension. Gels were stained with colloidal CBB G250. The identified proteins were listed in Tables 1, 2.