Table 2.

The differentially accumulated proteins identified in maize vp5 and Vp5 embryos associated to putative functions.

Spot	Protein name	UniProtKB accession	Protein homology by Blast (Score/Identity)	Abundance change folds	Subcellular location	Molecular function
PROTEINS IN INCREASED ABUNDANCE IN vp5 COMPARED TO Vp5
LEA proteins
1	Late embryogenesis abundant protein EMB564	P46517_MAIZE		13.1	Nucleus(Wu et al., 2013a)	Stress response
27	Uncharacterized protein	K7VM99_MAIZE	Group 3 LEA protein (1170/ 68.0%)	9.3	Cell wallb	Stress response
HSPs
2	17.8 kDa class II heat shock protein	P24632_MAIZE		2.4	Cytoplasma	Stress response; protein folding
3	16.9 kDa class I heat shock protein 1	B6T6N6_MAIZE		2.3	Nucleusb	Stress response; protein processing in ER
4	17.4 kDa class I heat shock protein 3	B6TLK8_MAIZE		2.4	Nucleusb	Stress response; protein folding; protein oligomerization
5	Heat shock protein 17.9	B6TDB5_MAIZE		2.5	Nucleusb	Stress response
6	Heat shock protein 17.2	Q43701_MAIZE		2.5	Nucleusb	Stress response; protein processing in ER
7	17.0 kDa class II heat shock protein	Q08275_MAIZE		2.1	Cytoplasma	Stress response; protein processing in ER
8	22.0 kDa class IV heat shock protein	B6TG53_MAIZE		2.4	Plastidb	Stress response; protein processing in ER
9	22.0 kDa class IV heat shock protein	B6TXB5_MAIZE		1.5	Plastidb	Stress response; protein processing in ER
24	17.4 kDa class I heat shock protein 3	B4F976_MAIZE		2.8	Nucleusb	Stress response; protein processing in ER
Oxidoreductase
10	1-Cys peroxiredoxin PER1	A2SZW8_MAIZE		7.3	Nucleusa	Phenylalanine metabolism; biosynthesis of other secondary metabolites
11	Short-chain dehydrogenase/reductase SDR family protein	B4FNZ9_MAIZE		3.6	Plastidb	Oxidation-reduction process
12	UDP-glucose 6-dehydrogenase	B7ZYX8_MAIZE		2.2	Plastidb	Cell wall pectin metabolic process
Others
25	Uncharacterized protein	B4FFK9_MAIZE	Lipoprotein-like (1104/ 81.0%)	3.1	Plastidb	Unknown
26	Uncharacterized protein	B4FFK9_MAIZE	Lipoprotein-like (1104/ 81.0%)	2.1	Plastidb	Unknown
PROTEINS IN REDUCED ABUNDANCE IN vp5 COMPARED TO Vp5
LEA proteins
13	Late embryogenesis abundant protein D-34	B6UH67_MAIZE		2.2	Nucleusb	Stress response
14	Late embryogenesis abundant protein D-34	B6UH67_MAIZE		3.9	Nucleusb	Stress response
15	Late embryogenesis abundant protein D-34	B6SNS4_MAIZE		3.1	Nucleusb	Stress response
16	late embryogenesis abundant protein D-34	B6SN63_MAIZE		2.4	Nucleusb	Stress response
22	Late embryogenesis abundant protein Lea14-A	B6UH99_MAIZE		1.8	Plastidb Nucleusb Golgi apparatusb	Stress response
29	Late embryogenesis abundant protein, group 3	Q42376_MAIZE		2.2	Cell wallb	Stress response
Enzymes
17	Short-chain dehydrogenase/reductase SDR family protein	B4FNZ9_MAIZE		1.7	Plastidb	Oxidation-reduction process
18	Short-chain dehydrogenase/reductase SDR family protein	B4FNZ9_MAIZE		2.0	Plastidb	Oxidation-reduction process
20	Thioredoxin	B4FH44_MAIZE		2.3	Cytoplasmb	Cell redox homeostasis; glycerol ether metabolic process; oxidation-reduction process
21	Glyoxalase family protein superfamily	B6SGF3_MAIZE		3.4	Cytoplasmb	Methylglyoxal metabolic process
23	Uncharacterized protein	C0HII8_MAIZE	Bowman-Birk serine protease inhibitor family protein (365/ 40.0%)	6.5	Extracellular regiona	Negative regulation of endopeptidase activity
Storage proteins
30	Globulin-1 S allele	B6UGJ0_MAIZE		3.6	Cell walla	Seed maturation
31	Globulin-1 S allele	B6UGJ0_MAIZE		2.7	Cell walla	Seed maturation
Others
19	Hypothetical protein ZEAMMB73_326753	K7UCT3_MAIZE	Seed maturation protein (152/ 52.0%)	6.8	Nucleusb	Unknown
28	Uncharacterized protein	B4FFK9_MAIZE	Lipoprotein-like (1104/ 81.0%)	2.7	Plastidb	Unknown

^aSubcellular location of proteins was annotated in UniProtKB/Swiss-Prot (http://www.expasy.org/).

^bSubcellular location of proteins was predicted using the online Plant-mPLoc server (http://www.csbio.sjtu.edu.cn/bioinf/plant-multi/).