Table 2.
Spot | Protein name | UniProtKB accession | Protein homology by Blast (Score/Identity) | Abundance change folds | Subcellular location | Molecular function |
---|---|---|---|---|---|---|
PROTEINS IN INCREASED ABUNDANCE IN vp5 COMPARED TO Vp5 | ||||||
LEA proteins | ||||||
1 | Late embryogenesis abundant protein EMB564 | P46517_MAIZE | 13.1 | Nucleus(Wu et al., 2013a) | Stress response | |
27 | Uncharacterized protein | K7VM99_MAIZE | Group 3 LEA protein (1170/ 68.0%) | 9.3 | Cell wallb | Stress response |
HSPs | ||||||
2 | 17.8 kDa class II heat shock protein | P24632_MAIZE | 2.4 | Cytoplasma | Stress response; protein folding | |
3 | 16.9 kDa class I heat shock protein 1 | B6T6N6_MAIZE | 2.3 | Nucleusb | Stress response; protein processing in ER | |
4 | 17.4 kDa class I heat shock protein 3 | B6TLK8_MAIZE | 2.4 | Nucleusb | Stress response; protein folding; protein oligomerization | |
5 | Heat shock protein 17.9 | B6TDB5_MAIZE | 2.5 | Nucleusb | Stress response | |
6 | Heat shock protein 17.2 | Q43701_MAIZE | 2.5 | Nucleusb | Stress response; protein processing in ER | |
7 | 17.0 kDa class II heat shock protein | Q08275_MAIZE | 2.1 | Cytoplasma | Stress response; protein processing in ER | |
8 | 22.0 kDa class IV heat shock protein | B6TG53_MAIZE | 2.4 | Plastidb | Stress response; protein processing in ER | |
9 | 22.0 kDa class IV heat shock protein | B6TXB5_MAIZE | 1.5 | Plastidb | Stress response; protein processing in ER | |
24 | 17.4 kDa class I heat shock protein 3 | B4F976_MAIZE | 2.8 | Nucleusb | Stress response; protein processing in ER | |
Oxidoreductase | ||||||
10 | 1-Cys peroxiredoxin PER1 | A2SZW8_MAIZE | 7.3 | Nucleusa | Phenylalanine metabolism; biosynthesis of other secondary metabolites | |
11 | Short-chain dehydrogenase/reductase SDR family protein | B4FNZ9_MAIZE | 3.6 | Plastidb | Oxidation-reduction process | |
12 | UDP-glucose 6-dehydrogenase | B7ZYX8_MAIZE | 2.2 | Plastidb | Cell wall pectin metabolic process | |
Others | ||||||
25 | Uncharacterized protein | B4FFK9_MAIZE | Lipoprotein-like (1104/ 81.0%) | 3.1 | Plastidb | Unknown |
26 | Uncharacterized protein | B4FFK9_MAIZE | Lipoprotein-like (1104/ 81.0%) | 2.1 | Plastidb | Unknown |
PROTEINS IN REDUCED ABUNDANCE IN vp5 COMPARED TO Vp5 | ||||||
LEA proteins | ||||||
13 | Late embryogenesis abundant protein D-34 | B6UH67_MAIZE | 2.2 | Nucleusb | Stress response | |
14 | Late embryogenesis abundant protein D-34 | B6UH67_MAIZE | 3.9 | Nucleusb | Stress response | |
15 | Late embryogenesis abundant protein D-34 | B6SNS4_MAIZE | 3.1 | Nucleusb | Stress response | |
16 | late embryogenesis abundant protein D-34 | B6SN63_MAIZE | 2.4 | Nucleusb | Stress response | |
22 | Late embryogenesis abundant protein Lea14-A | B6UH99_MAIZE | 1.8 | Plastidb Nucleusb Golgi apparatusb | Stress response | |
29 | Late embryogenesis abundant protein, group 3 | Q42376_MAIZE | 2.2 | Cell wallb | Stress response | |
Enzymes | ||||||
17 | Short-chain dehydrogenase/reductase SDR family protein | B4FNZ9_MAIZE | 1.7 | Plastidb | Oxidation-reduction process | |
18 | Short-chain dehydrogenase/reductase SDR family protein | B4FNZ9_MAIZE | 2.0 | Plastidb | Oxidation-reduction process | |
20 | Thioredoxin | B4FH44_MAIZE | 2.3 | Cytoplasmb | Cell redox homeostasis; glycerol ether metabolic process; oxidation-reduction process | |
21 | Glyoxalase family protein superfamily | B6SGF3_MAIZE | 3.4 | Cytoplasmb | Methylglyoxal metabolic process | |
23 | Uncharacterized protein | C0HII8_MAIZE | Bowman-Birk serine protease inhibitor family protein (365/ 40.0%) | 6.5 | Extracellular regiona | Negative regulation of endopeptidase activity |
Storage proteins | ||||||
30 | Globulin-1 S allele | B6UGJ0_MAIZE | 3.6 | Cell walla | Seed maturation | |
31 | Globulin-1 S allele | B6UGJ0_MAIZE | 2.7 | Cell walla | Seed maturation | |
Others | ||||||
19 | Hypothetical protein ZEAMMB73_326753 | K7UCT3_MAIZE | Seed maturation protein (152/ 52.0%) | 6.8 | Nucleusb | Unknown |
28 | Uncharacterized protein | B4FFK9_MAIZE | Lipoprotein-like (1104/ 81.0%) | 2.7 | Plastidb | Unknown |
Subcellular location of proteins was annotated in UniProtKB/Swiss-Prot (http://www.expasy.org/).
Subcellular location of proteins was predicted using the online Plant-mPLoc server (http://www.csbio.sjtu.edu.cn/bioinf/plant-multi/).