Figure 5.

Expression levels of RD29B, EM6, and ABF4 genes in Col-0, Atgrdp2-1, and 35S::AtGRDP2-OE3 overexpression line during salt treatment. Fourteen days old plants grown on 0.5X MS plates were transferred to 0.5X MS medium containing 0, 125, and 150 mM NaCl, for 1 and 3 days. Ten seedlings of each line per triplicate were used. The expression levels were estimated by qRT-PCR with the respective three technical replicates using SYBR green dye. Normalized fold change was calculated comparing the target gene expression (under salt stress) with a control (untreated Col-0), after normalization to the Arabidopsis UBQ5 gene using the (2^−ΔΔCt) method. In case of ratios lower than 1, the inverse of the ratio was estimated and the sign was changed.