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. 2015 Jan 20;8:463. doi: 10.3389/fncel.2014.00463

Figure 2.

Figure 2

ANAVEX2-73 (AN2-73) and PRE-084 (PRE) protected against Aβ25–35-induced alteration of mitochondrial respiration in mice. Mice were treated with ANAVEX2-73 (0.3 mg/kg IP), PRE-084 (0.5 mg/kg IP) or vehicle, before injection of Sc. Aβ or Aβ25–35 (9 nmol ICV). (A–C) Mitochondria (0.8 mg/ml) were loaded in the chamber with appropriate buffer at 30°C. State 2 respiration was activated by addition of pyruvate-malate (5 mM). State 3 respiration was induced with ADP. State 4 respiration was provoked by the addition of carboxyatractyloside (CAT), a blocker of ATP-ADP carrier. Finally, addition of tyrphostine, an uncoupling agent, activated the uncoupled respiration. The respiratory control ratio is the state 3/state 4 ratio. n = 4–8 per group, F(5,38) = 7.11, p < 0.0001 for state 2 and F(5,38) = 8.43, p < 0.0001 for state 4 in (A); F(5,38) = 13.1, p < 0.0001 for state 3 and F(5,38)= 8.43, p < 0.0001 for state 3 in (B); F(5,38) = 2.58, p < 0.05 in (C). *p < 0.05, **p < 0.01 vs. Sc. Aβ/Veh; #p < 0.05, ##p < 0.01, ###p < 0.001 vs. Aβ25–35/Veh. Dunnett’s test. (D) Typical trace of O2 consumption for Sc. Aβ and Aβ25–35-injected mice.