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. 2015 Jan 19;56(1):505–514. doi: 10.1167/iovs.14-15539

Figure 7.

Figure 7

Localization of JNK3 protein in the cytoplasm of RGCs. Twenty-four hours after intravitreal injection of PBS or Poly(I:C), eyes were treated with an intravitreal injection of MitoTracker Red. Twenty-four hours after MitoTracker Red treatment, retinas isolated from PBS- or Poly(I:C)-treated eyes were immunostained with antibodies against JNK3 and flat mounted. Results presented indicate that MitoTracker Red labeled the mitochondria in RGCs and in cells in the deeper layers of the retina in both PBS- and Poly(I:C)-treated eyes. In PBS-treated eyes, low levels of JNK3 protein were localized in RGCs, but JNK3 protein was not colocalized with MitoTracker Red. In addition, after Poly(I:C) treatment, JNK3 protein levels were upregulated in RGCs, but elevated levels of JNK3 protein was not colocalized with MitoTracker Red. Images were obtained at ×40 magnification. Scale bar: 25 μm.