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. 2015 Jan 19;56(1):505–514. doi: 10.1167/iovs.14-15539

Figure 8.

Figure 8

Effect of TLR3 inhibitor on JNK3 and pJNK3 protein levels in the retina. Forty-eight hours after mouse eyes were treated with PBS or Poly(I:C) with or without TLR3 inhibitor, total proteins were isolated from the retinas, and aliquots containing an equal amount of retinal proteins (50 μg) were subjected to Western blot analysis using antibodies against JNK3 and pJNK3. Actin Western bands indicate loading controls of total proteins. Bar graphs indicate semiquantitative analysis of JNK3 and pJNK3 protein from the Western blots. Results presented indicate that TLR3 inhibitor did not affect low levels of JNK3 and pJNK3 proteins observed in PBS-treated eyes (A, B). In contrast, Poly(I:C)-mediated upregulation of JNK3 and pJNK3 proteins (C, D) was reduced in eyes treated with Poly(I:C) along with 10 μM concentration of TLR3 inhibitor, but not with 5 μM. *P < 0.05 when JNK3 and pJNK3 protein levels were compared with PBS-treated eyes. **P < 0.05 when JNK3 and pJNK3 protein levels were compared with Poly(I:C)-treated eyes. NS, not significant.