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. 2014 Dec 10;15(1):1084. doi: 10.1186/1471-2164-15-1084

Table 4.

Forward and reverse primers used for qPCR validation of RNA-seq

Gene Forward primer Reverse primer
SPSB4 5′ AATGGACTTGACCCGGAAC 3′ 5′ TTTCAGAGACAGAGGCAAAGG 3′
TRPC5 5′ CTGCCCTGGGTTCTAGGTTT 3′ 5′ GGGAGTTCATTGCAAAATCC 3′
BNC1 5′ TGGATATGTGCTGCAGGATG 3′ 5′ TGCCATTAACTCCACAATGG 3′
ANGPTL4 5′ TGTGACATGACTGCAGAAGG 3′ 5′ CAGCCAGAAGTCACCATGAA 3′
LIMS2 5′ CAGATGGGCTTTTCTATGAGTT 3′ 5′ GAAACATTCTGGGTGCCAGT 3′
ERC2 5′ GTCTTGCCTCAACACAGCAA 3′ 5′ GGCAATGTTTGCATCCTTTT 3′
S100A1 5′ AGCTGAGCAAGAAGGAGCTG 3′ 5′ GGTCCTGCATGATCTTCTCC 3′
FGF14 5′ TACCCAAGCCATTGGAAGTT 3′ 5′ GTTTGCCGCCATTCATTATT 3′
DIO3 5′ GCTCTCTTCCTTCGGGATCT 3′ 5′ CCCATTTCAAAATCGGTCAT 3′