Figure 2. TIS efficiency determined by FACS-seq.

1. Individual FACS-seq histograms spanning a range of translation efficiency values. The background color corresponds to the median translation initiation efficiency. The TIS sequences are indicated above each histogram.
2. Heat map of entire TIS sequence space. The sequences were arranged to highlight trends in TIS strength. The +4/+5 positions were not labeled, but they follow the same nucleotide order of U/C/A/G.