Table 3.
The effects of short-term (6 day) BA or GH treatment of lambs at 60 or 120 days of age on MyHC isoform mRNA expression in different muscles
| D60 | D120 | ||||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|
| Muscle | Isoform (%) | CO | BA | GH | s.e.d. | P-value | CO | BA | GH | s.e.d. | P-value |
| n | 10 to 11 | 10 | 10 | 10 to 11 | 10 | 9 to 10 | |||||
| Longissimus dorsi | MyHCI | 7.2 | 3.9 a | 6.6 | 0.8 | <0.001 | 6.8 | 5.0 | 8.1 | 1.9 | 0.277 |
| MyHCIIA | 26.9 | 7.0 a | 34.1 | 3.4 | <0.001 | 21.1 | 5.2 a | 25.4 | 3.6 | <0.001 | |
| MyHCIIX/IIB | 65.9 | 89.1 a | 59.3 | 3.6 | <0.001 | 72.1 | 89.8 a | 66.5 | 4.2 | <0.001 | |
| Supraspinatus | MyHCI | 34.7 | 37.7 | 42.3 | 8.8 | 0.693 | 56.6 | 51.6 | 45.5 | 9.6 | 0.520 |
| MyHCIIA | 21.2 | 9.1 a | 27.9 | 3.8 | <0.001 | 15.8 | 5.3 a | 17.2 | 2.1 | <0.001 | |
| MyHCIIX/IIB | 44.1 | 53.2 | 29.8 | 7.3 | 0.012 | 27.6 | 43.1 | 37.3 | 9.1 | 0.243 | |
| Semitendinosus | MyHCI | 13.5 | 8.9 | 11.7 | 2.4 | 0.161 | 11.2 | 14.6 | 13.5 | 2.2 | 0.259 |
| MyHCIIA | 20.6 | 4.3 a | 25.5 | 2.8 | <0.001 | 17.1 | 2.7 a | 20.4 | 2.6 | <0.001 | |
| MyHCIIX/IIB | 66.0 | 86.8 a | 62.7 | 4.0 | <0.001 | 71.7 | 82.7 a | 66.2 | 3.8 | <0.001 | |
BA=β agonist; GH=growth hormone; MyHC=myosin heavy chain; CO=control.
a
Significantly different to control (Dunnett’s test; P<0.05).