Table 6.
The effects of short-term (6 day) BA or GH treatment of lambs at 60 or 120 days of age, on mRNA expression of regulators of muscle contractile and metabolic properties in the Longissimus dorsi
| D60 | D120 | |||||||||
|---|---|---|---|---|---|---|---|---|---|---|
| Gene mRNA target a | CO | BA | GH | s.e.d. | P-value | CO | BA | GH | s.e.d. | P-value |
| n | 11 | 10 | 10 | 11 | 10 | 10 | ||||
| PPARα | 4.46 | 3.82 | 3.94 | 0.31 | 0.096 | 4.53 | 3.74 | 2.91 b | 0.35 | <0.001 |
| PPARδ | 4.30 | 5.75 | 4.08 | 0.86 | 0.124 | 3.82 | 5.14 b | 4.22 | 0.35 | 0.002 |
| PPARγ | 4.85 | 2.91 | 4.39 | 1.76 | 0.516 | 4.46 | 3.45 | 4.14 | 0.48 | 0.111 |
| PGC1α | 4.53 | 4.36 | 3.70 | 0.77 | 0.521 | 4.28 | 4.67 | 3.85 | 0.58 | 0.376 |
| PGC1β | 5.11 | 2.87 b | 3.85 b | 0.45 | <0.001 | 4.16 | 2.68 b | 3.42 | 0.44 | 0.007 |
| RIP140 | 3.59 | 5.58 b | 4.30 | 0.66 | 0.015 | 3.86 | 5.63 b | 3.98 | 0.55 | 0.005 |
| Six1 | 4.31 | 2.58 b | 4.11 | 0.41 | <0.001 | 4.66 | 2.24 b | 4.24 | 0.27 | <0.001 |
| Eya1 | 4.38 | 2.73 b | 4.29 | 0.53 | 0.006 | 5.35 | 2.93 b | 5.04 | 0.69 | 0.002 |
| MyoD | 2.95 | 2.66 | 2.73 | 0.40 | 0.735 | 3.00 | 2.01 b | 3.28 | 0.39 | 0.008 |
| Myogenin | 3.34 | 4.65 b | 3.65 | 047 | 0.023 | 3.15 | 4.54 b | 3.23 | 0.40 | 0.002 |
| MRF4 | 3.02 | 4.47 | 3.59 | 1.36 | 0.552 | 4.08 | 4.14 | 5.06 | 0.63 | 0.230 |
| Myf-5 | 2.71 | 3.69 | 3.22 | 0.40 | 0.059 | 4.33 | 3.71 | 3.71 | 0.57 | 0.436 |
BA=β agonist; GH=growth hormone; CO=control; PPAR=peroxisome proliferator-activated receptor; PGC=PPAR-γ coactivator; RIP=receptor-interacting protein; MRF=myogenic regulatory factor.
a
Values for gene targets expressed as arbitrary units per unit total RNA.
b
Significantly different to control (Dunnett’s test; P<0.05).