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. 2014 Dec 12;15(1):1097. doi: 10.1186/1471-2164-15-1097

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© Klasson et al.; licensee BioMed Central. 2014

This article is published under license to BioMed Central Ltd. This is an Open Access article distributed under the terms of the Creative Commons Attribution License (http://creativecommons.org/licenses/by/4.0), which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly credited. The Creative Commons Public Domain Dedication waiver (http://creativecommons.org/publicdomain/zero/1.0/) applies to the data made available in this article, unless otherwise stated.

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Confirmation of coverage metrics using a qPCR based assay. The copy number was confirmed and found to be accurate up to 8 copies per haploid genome using a qPCR assay measuring the ratio of the LGT abundance to that of six single copy Drosophila genes as shown for the 3 kbp mate pair library for the cured Hawaii (Panel A), India (Panel B), and Indonesia (Panel C). A significant positive correlation (p < 0.001, linear regression) is found for the cured Hawaii and the India line The results in Panels A and C represent the accumulation of multiple qPCR experiments while those in Panel B result from a single experiment.