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. 2014 Dec 12;15(1):1097. doi: 10.1186/1471-2164-15-1097

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© Klasson et al.; licensee BioMed Central. 2014

This article is published under license to BioMed Central Ltd. This is an Open Access article distributed under the terms of the Creative Commons Attribution License (http://creativecommons.org/licenses/by/4.0), which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly credited. The Creative Commons Public Domain Dedication waiver (http://creativecommons.org/publicdomain/zero/1.0/) applies to the data made available in this article, unless otherwise stated.

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Fluorescence in situ hybridization of mitotic chromosomes. Mitotic chromosomes were hybridized with a Wolbachia-specific WRi_004290 probe amplified from sequence-verified plasmids and visualized at 60X. While hybridization was not detected in polytene chromosomes with WRi_004290, it is detected in the mitotic chromosomes. The fluorescence appears to be localized to the centromere of the abnormally large fourth chromosomes of D. ananassae. The larger 2^nd and 3^rd chromosomes are visualized nearby as well as a likely X chromosome. Hybridization to the fourth chromosome is consistent with the LGT being largely heterochromatic.