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. 2014 Dec 21;14:45. doi: 10.1186/s12861-014-0045-6

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© Forsthoefel et al.; licensee BioMed Central. 2014

This is an Open Access article distributed under the terms of the Creative Commons Attribution License (http://creativecommons.org/licenses/by/4.0), which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly credited. The Creative Commons Public Domain Dedication waiver (http://creativecommons.org/publicdomain/zero/1.0/) applies to the data made available in this article, unless otherwise stated.

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Methanol bleaching abolishes labeling by mAb 3F11. (A) mAb 3F11 does not label samples bleached in methanol (12-16 hr). (B) mAb 3F11 labels the intestine in formaldehyde-fixed animals bleached in PBS (12-16 hr). Mg treatment also substantially improves 3F11 labeling in HCl-treated samples, but substantially reduces labeling in NAc-treated samples (B), demonstrating that some antibodies are uniquely sensitive to specific combinations of treatments. In all panels, anterior is to the left. Scale bars: 500 μm.