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. 2014 Nov 3;10(11):756. doi: 10.15252/msb.20145422

Figure 4. ede1 mutants lacking the coiled coil domain are mislocalized and cannot oligomerize.

Figure 4

  • A Schematic overview of the domain structure of Ede1 and ede1 mutants with proposed interaction sites with endocytic adaptors. Domain sizes are schematic and do not exactly match the actual length. The corresponding fluorescence images are shown on the right. Scale bar corresponds to 2 μm.
  • B–D Comparison of the cytoplasmic concentration (B), the normalized brightness per particle per second (C), and the cytoplasmic diffusion coefficient of Ede1 and its truncation mutants (D). Measurements were performed in 12–44 individual cells. Error bars represent standard deviation (***P-value ≤ 0.001; *P-value ≤ 0.05; ns, not significant).