A–D y-axis shows the ratio of antisense-to-sense levels relative to ratio
in 968 strain (log scale). Error bars indicate the standard error to the mean among the three
biological replicates. Analysis was performed for four genes: cdb4 (A),
SPAC29E6.10c (B), its3 (C), paf1 (D) in seven
different strains: the two parental strains (968, Y0036), two pools of strains corresponding to all
the segregants separated according to their genotype at the swc5 locus, and strains
deleted for swc5, pht1, and both pht1 and
clr4. No difference was observed between the parents in cdb4,
which indicates that other genetic events are implicated, as expected since cdb4 is
also associated with another aseQTL (Supplementary Information). For paf1 and
its3, effects observed in swc5-fs strains (pool and Y0036) were
not observed in Δswc5. This may be due to other genetic effects in
cis of swc5, or to effects of auxotrophic and mating makers which
differ between 968 and Δswc5 (Supplementary Information).
