Figure 8. Organization of coding genes whose antisense expression is linked to the swc5 locus.

The organization of gene pairs in the genome is either convergent, divergent, or in tandem and overlapping or not. However, the distinction between overlapping and non-overlapping genes is questionable in Schizosaccharomyces pombe, because of imprecise definition of the untranslated regions (UTRs). Scheme on the left shows geometry of gene pair orientations and the potential antisense RNA (represented in red wavy lines). The number of swc5 aseQTL targets falling into each category is shown (green; observed). We used an empirical distribution based on 1,000,000 random assignments of antisense swc5 targets (gray; expected), to test for enrichment or depletion of each category (*P < 10⁻⁶). The depletion of tandem gene pairs is due to the non-random organization of the S. pombe genome (Supplementary Information, Supplementary Fig S17). Error bars indicate standard deviation over 1,000,000 permutations.