Figure 7.

miR-30 regulates matrix metalloproteinase (MMP) 19 expression in A549 cells. (A) Expression of miR-30a, -30d, and -30e were down-regulated in human non–small cell lung cancer compared with corresponding normal control subjects as detected by Taqman quantitative reverse-transcriptase polymerase chain reaction (*P < 0.05; n = 15). (B) MMP19 gene expression was determined by reverse-transcriptase polymerase chain reaction in A549 cells 24 hours following transfection with antagomiR-30e oligonucleotides or controls. Data represent the mean ± SEM (*P < 0.05, n = 4). (C) MMP19 protein expression was determined by immunoblotting lysates of A549 cells 48 hours following transfection with antagomiR-30e oligonucleotides or control. (D) Quantification of MMP19 band intensity in C in ImageJ. Data represent mean ± SEM (*P < 0.05, n = 3).