HIV+COPD+ patients have a significantly higher
surface expression of CD95+ and/or programed death
(PD)-1+ on lung mucosal CD4+ T cells.
(A) Representative flow cytometric plots of the lung
mononuclear cells (LMNC)
CD4+CD95+PD-1+ for
HIV+COPD− (left) versus
HIV+COPD+ (middle), and
HIV−COPD+ (right)
patient. (B) Pooled data showing cumulative frequencies of
LMNC CD4+CD95+ for 11
HIV+COPD−, 12
HIV+COPD+, and 7
HIV−COPD+ patients. (C)
Pooled data showing cumulative frequencies of LMNC
CD4+CD95+PD-1+ for 11
HIV+COPD− patients (white
bars), 12 HIV+COPD+ (black
bars), and 7 HIV−COPD+
(gray bars) patients. Values represent mean ± SEM, and
P values were calculated using the Mann-Whitney
t test. (D) Scatterplot analysis of LMNC
Pol- and Gag-specific CD4+IFNγ+ responses
and CD4+CD95+PD-1+ frequencies.
Values are for 12 HIV+COPD+ (black
circles) and 11 HIV+COPD−
(white circles) individuals in the cohort. Analysis was
performed using the Spearman rho test.