Figure 4.

HIV⁺COPD⁺ patients have a significantly higher surface expression of CD95⁺ and/or programed death (PD)-1⁺ on lung mucosal CD4⁺ T cells. (A) Representative flow cytometric plots of the lung mononuclear cells (LMNC) CD4⁺CD95⁺PD-1⁺ for HIV⁺COPD⁻ (left) versus HIV⁺COPD⁺ (middle), and HIV⁻COPD⁺ (right) patient. (B) Pooled data showing cumulative frequencies of LMNC CD4⁺CD95⁺ for 11 HIV⁺COPD⁻, 12 HIV⁺COPD⁺, and 7 HIV⁻COPD⁺ patients. (C) Pooled data showing cumulative frequencies of LMNC CD4⁺CD95⁺PD-1⁺ for 11 HIV⁺COPD⁻ patients (white bars), 12 HIV⁺COPD⁺ (black bars), and 7 HIV⁻COPD⁺ (gray bars) patients. Values represent mean ± SEM, and P values were calculated using the Mann-Whitney t test. (D) Scatterplot analysis of LMNC Pol- and Gag-specific CD4⁺IFNγ⁺ responses and CD4⁺CD95⁺PD-1⁺ frequencies. Values are for 12 HIV⁺COPD⁺ (black circles) and 11 HIV⁺COPD⁻ (white circles) individuals in the cohort. Analysis was performed using the Spearman rho test.