Figure 3.

Comparison of dexamethasone-induced glucocorticoid receptor (GR) phosphorylation at serine 211. (A) Time-dependent, dexamethasone-induced, phosphorylation of GR Ser211. After serum starvation for 24 hours, airway smooth muscle cells (ASMC) of healthy subjects were treated with dexamethasone (10⁻⁶ M) for times indicated. A representative blot is shown. Bars represent mean ± SEM from three ASMC of healthy subjects. **P < 0.01, ***P < 0.001 versus unstimulated (US). (B) Comparison of GR Ser211 phosphorylation. ASMC of healthy subjects and patients with nonsevere and severe asthma were treated with dexamethasone (10⁻⁶ M) for 2 hours. Phosphorylated (Ser211) and total GR protein in whole-cell lysates were measured by Western blot followed by densitometric analysis. Horizontal lines represent median.