Skip to main content
. 2015 Jan 1;191(1):54–62. doi: 10.1164/rccm.201402-0314OC

Figure 4.

Figure 4.

Impaired dexamethasone mediated attenuation of tumor necrosis factor (TNF)-α–induced p65 recruitment to the CCL11 promoter in airway smooth muscle cells (ASMC) of patients with severe asthma. After serum starvation for 24 hours, ASMC were pretreated with dexamethasone (10−7 M) for 2 hours and stimulated with TNF-α (10 ng/ml) for 1 hour. p65 recruitment to the CCL11 promoter in ASMC of (A) healthy subjects and (B) patients with nonsevere and (C) severe asthma was measured by chromatin immunoprecipitation assay. Bars represents mean ± SEM. Dex = dexamethasone; NS = not significant; US = unstimulated. *P < 0.05, ***P < 0.001.