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. 2014 Dec 15;190(12):1342–1354. doi: 10.1164/rccm.201406-1150CI

Figure 3.

Figure 3.

Effects of hematogenous organophosphorus (OP) and aspirated OP on minipig lung. Comparison of lung architecture in anesthetized minipigs 48 hours after administration of saline into the lung (control pig; A, D, and G), gastric contents and the agricultural OP insecticide dimethoate EC40 into the contralateral lung (indirect hematogenous injury; B, E, and H), and gastric contents and agricultural OP insecticide dimethoate EC40 into the right lung (direct injury; C, F, I). (A–C) Light microscopy images (original magnification: ×10–20) with hematoxylin and eosin. Compared with indirect injury, direct injury caused greater alveolar and interstitial edema, neutrophil infiltration, hemorrhage, fibrin deposition, vascular congestion, and necrosis. Images edited in PowerPoint. (D–F) Scanning electron microscopy images (original magnification: ×171–324) of the same lungs. Direct injury shows extensive destruction of the alveolar capillary framework, with fibrin mesh and clot formation. (G–I) Transmission electron microscopy images (original magnification: ×25,000) of the same lungs. Both indirect and direct injury cause alveolar capillary membrane swelling. The black arrow signifies the alveolar capillary membrane in control (G) and indirect (H) lungs. After direct injury, this has led to the alveolar epithelium peeling away into the alveolar space and fibrin deposition (red arrow) in and around the alveolar capillary membrane.