Figure 4. Regulation of H⁺-ATPase activity is required for lateral auxin gradient formation.

1. DII-Venus signal in hypocotyls was examined before (left) or after (right) 1 h blue light (BL) irradiation with a fluence rate of 10 μmol m⁻² s⁻¹.
2. General activation of H⁺-ATPases and inhibition of auxin transport prevent auxin gradient formation. Seedlings treated with DMSO, NPA, or FC were analyzed as in (A). Quantification of DII-Venus signal was performed on 11–13 seedlings for each treatment, and data represent the ratio of the DII-Venus fluorescence signal between the lit side and the shaded side. Values are means, and error bars represent standard errors.

Source data are available online for this figure.