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. Author manuscript; available in PMC: 2015 Jan 20.
Published in final edited form as: RSC Adv. 2014;9:4334–4342. doi: 10.1039/C3RA46839A

Fig. 2.

Fig. 2

Capture efficiency experiments. (A) Flow rate and fixation affects capture efficiencies of MCF-7 cells spiked into blood or PBS, either fixed or unfixed. Samples were run at 1 mL min−1 (dark gray), 5 mL min−1 (light gray), or 10 mL min−1 (white) (upper left panel) (n = 3). *All 3 filters clogged with blood clots (B) Capture efficiencies of MCF-7 cells using porosity microfilters designs of 70 000 (diamond), 110 000 (square) or 160 000 (triangle) and pore diameters from 5–9 μm (lower panel, n = 3). (C) Capture efficiency of 5 cell lines using the 160 000 pore design, compared by spiking enumerated cells into blood with Prefixation Buffer (gray), or 1XPBS (white) (upper right panel) (n = 3; ~50 spiked cells).