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Primer sequences, product length, and CpG units used for MassARRAY.
| Primer number |
Forward primer (5′-3′)1 | Reverse primer (5′-3′)2 | Product length (bp) |
CpG unit |
|---|---|---|---|---|
| SQ1 | TTAAGGATTTGTTTGTATTTAATTGG | CAAACAATACCACAATTTATATCTTTTA | 416 | 6 |
| SQ2 | AGATTTGGAGAGATGGTTTAGTGGT | ACCCATTTCCTACAAAAAAATATCC | 498 | 10 |
| SQ3 | TTAAAAGATATAAATTGGGGTTGGG | ACCAACAAAACCCCTCTAAATAAAC | 349 | 9 |
1For 10-mer tag, CAGTAATACGACTCACTATAGGGAGAAGG was added and 2for T7 promoter tag AGGAAGAGAG was added.
