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. 2014 Dec 6;14:105. doi: 10.1186/s12896-014-0105-9

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© Yamaguchi et al.; licensee BioMed Central. 2014

This is an Open Access article distributed under the terms of the Creative Commons Attribution License (http://creativecommons.org/licenses/by/4.0), which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly credited. The Creative Commons Public Domain Dedication waiver (http://creativecommons.org/publicdomain/zero/1.0/) applies to the data made available in this article, unless otherwise stated.

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Mesenchymal stem cell spheroid, generated from rat mesenchymal stem cell lines. (A) Parental cell line grown permanently as a monolayer culture. (B) and (C) The generation of mesenchymal stem cell (MSC) spheroids. Cellular aggregation of MSCs was observed 2 h after a microplate culture (B). A cell aggregation formed a compact spheroid at 24 h (C). Scale bars = 100 μm (a and c) and 1 mm (B).