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SEC-MALS analysis of the purified HisDapGalNAcT2 protein. (A) Light scattering spectrum of the eluted glycosyltransferase fraction in 50 mM Tris, 150 mM NaCl, pH 8. The orange line across the peak at the retention time of 17 min shows the molar mass distribution. (B) The UV-spectrum measured at 280 nm shows a single peak representing monomers of HisDapGalNAcT2.
