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. 2015 Jan 13;14:3. doi: 10.1186/s12934-014-0186-0

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© Lauber et al.; licensee BioMed Central. 2015

This is an Open Access article distributed under the terms of the Creative Commons Attribution License (http://creativecommons.org/licenses/by/4.0), which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly credited. The Creative Commons Public Domain Dedication waiver (http://creativecommons.org/publicdomain/zero/1.0/) applies to the data made available in this article, unless otherwise stated.

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ESI-MS/MS analysis of non-glycosylated and glycosylated Filgrastim™ (G-CSF). (A) Amino acid sequence of the substrate Filgrastim™ showing the tryptic digest peptides detected by ESI-MS/MS (yellow highlight, 58% coverage) and post-translationally modified amino acids (e.g. oxidation, green). G-CSF peptides detected by ESI-MS/MS after treatment with glycosyltransferases (B) HisDapGalNAcT2 (42% coverage) or (C) of rhGalNAcT2 (48% coverage), showing the glycosylation site at Thr¹⁶⁶ (red box and black arrow).