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. Author manuscript; available in PMC: 2015 Jan 20.
Published in final edited form as: J Comp Neurol. 2012 Mar 1;520(4):816–831. doi: 10.1002/cne.22791

Figure 3.

Figure 3

Putative neurite profiles in the wild-type zebrafish skin are absent in neurogenin-1 morphants and degenerate after sensory neuron ablation. A–D: TEM images of the zebrafish epidermis at 54 hpf (A,B) and 78 hpf (C,D). P, peridermal cell; B, basal cell; arrowheads point to basement membrane. Orientation for all EM images is periderm upward. A,C: Wild-type embryos at 54 (A) and 78 (C) hpf. Asterisks indicate neurite-like profiles. B,D: neurogenin-1 Morphant embryos at 54 (B) and 78 (D) hpf. No neurite-like profiles were observed. E,F: TEM images of the zebrafish epidermis at 79.5 hpf. All the cell bodies of one trigeminal ganglion were laser ablated at 78 hpf. Images were acquired ≥200 μm from the site of ablation. Red arrows point to degenerating axon membranes and organelles. Yellow asterisks indicate skin cell protrusions toward the degenerating axon. The red asterisk in E indicates a seemingly intact axon. Such degenerating axon profiles were not seen on the contralateral side of the head, where trigeminal innervation remained intact. Scale bars = 1 μm.