Table 1.
Parameters for identifying light-evoked monosynaptic A- and C-fibre-mediated responses
| A) DRG recordings | Large diameter neurons | Small diameter neurons |
|---|---|---|
| Action potential delay after onset of light pulse: | 2 – 4 ms | 2 – 8 ms |
| Mean action potential jitter at 0.2 Hz | ~ 1 ms | ~ 1 ms |
| B) Postsynaptic EPSCs: | A-fibre-mediated responses | C-fibre-mediated responses |
| Conduction velocity (as determined from response delay): | ≥ 1.5 m/s | ≤ 1.0 m/s |
| Failures upon repetitive stimulation: | No failures at 10 Hz | No failures at 1 Hz |
| Jitter upon 0.2 Hz stimulation: | Jitter minus 1 ms < 10% of EPSC latency | |
A, Table illustrating action potential delay and jitter upon photo-stimulation in A- and C-fibres, as measured in large and small diameter eYFP+ DRG neurons, respectively. B, Criteria used for classifying light-evoked EPSCs as monosynaptic A- or C-fibre-mediated responses.