Table 4.
miRNAs | Mean (SEM) of miRNA level * in patients with malignant SPNs | Mean (SEM) of miRNA level * in patients with benign SPNs | P-value | AUC (95% CI) | Sensitivity | Specificity |
---|---|---|---|---|---|---|
miR-205 | 85.66 (19.16) | 72.72 (64.82) | 0.0267 | 0.635 (0.552 to 0.719) | 59.74 | 53.93 |
miR-708 | 1.01 (0.17) | 4.86 (1.37) | 0.0048 | 0.656 (0.561 to 0.750) | 64.71 | 62.50 |
miR-375 | 698.81 (185.83) | 81.26 (23.80) | 0.0026 | 0.666 (0.594 to 0.756) | 66.23 | 62.22 |
miR-200b | 879.82 (228.67) | 49.10 (3.11) | 0.0013 | 0.679 (0.589 to 0.768) | 65.22 | 61.19 |
miR-182 | 43.95 (12.76) | 2.16 (0.31) | 0.0018 | 0.684 (0.611 to 0.778) | 64.94 | 59.76 |
miR-155 | 23.76(4.59) | 4.54 (0.59) | < 0.0001 | 0.697 (0.616 to 0.779) | 62.67 | 62.96 |
miR-372 | 136.49 (32.55) | 17.58 (3.56) | 0.0006 | 0.707 (0.628 to 0.786) | 63.64 | 60.98 |
miR-143 | 9.46 (1.87) | 1.86 (0.27) | 0.0002 | 0.723 (0.644 to 0.803) | 63.38 | 61.73 |
miR-486-5p | 29.46 (4.18) | 241.62 (49.80) | 0.0001 | 0.748 (0.674 to 0.821) | 74.03 | 66.67 |
miR-126 | 3.83 (1.38) | 78.88 (23.62) | 0.0030 | 0.777 (0.704 to 0.851) | 77.63 | 75.00 |
miR-31 | 2.73 (0.45) | 0.47 (0.07) | < 0.0001 | 0.789 (0.719 to 0.849) | 60.23 | 82.67 |
miR-21 | 53.60 (6.43) | 7.56 (1.39) | < 0.0001 | 0.819 (0.753 to 0.874) | 78.16 | 71.08 |
miR-210 | 67.28 (6.07) | 5.79 (0.42) | < 0.0001 | 0.853 (0.792 to 0.901) | 75.27 | 85.88 |
Combined three miRNAs† | < 0.0001 | 0.919 (0.864 to 0.956) | 82.93 | 87.84 |
Abbreviations: SEM, the standard error of the mean; AUC, the area under receiver operating characteristic curve; CI, confidence interval.
The relative expression of each miRNA was calculated by using 2−ΔΔ threshold cycle (Ct), in which, ΔCT were CT values normalized to miR-16 control gene.
The three miRNAs: miRs-21, 31, and 210.