Figure 3. Atg16L⁺ vesicles from Anxa2^−/− mice are deficient in PI and PS.

(a) FACS analysis of Atg16L⁺ vesicles in primary dendritic cells from Anxa2^+/+ and Anxa2^−/− mice. (b) Thin layer chromatography of cholesterol, PE and PS extracted from Atg16L⁺ vesicles sorted from dendritic cells of Anxa2^+/+ and Anxa2^−/− mice. (c) Thin layer chromatography of PI, PIP2 and PIP3 extracted from Atg16L⁺ vesicles sorted from Anxa2^+/+ and Anxa2^−/− dendritic cells. Data presented in a,b are representative of four separate experiments. (d) Mass spectrometry of lipids extracted from Atg16L⁺ vesicles sorted from Anxa2^+/+ and Anxa2^−/− dendritic cells; data are representative of four separate experiments.