Fig. 4.

Distribution of Mn porphyrin mimic in mitochondria (aqua bars) and cytosol (orange bars) isolated from wild-type S. cerevisiae grown in SDC for 24 h at 220 rpm in 30 °C with 10 µM MnTM-2-PyP⁵⁺ (TM), MnTE-2-PyP⁵⁺ (TE), MnTnBu-2-PyP⁵⁺ (Bu), or MnTnHex-2-PyP⁵⁺ (Hex). Each mimic distribution value displayed was determined using measurements from at least two trials, with error bars representing standard deviation. Concentrations of the Mn porphyrin mimics were measured using high-pressure liquid chromatography and then normalized to protein concentrations determined via Bradford assays in the respective mitochondrial and cytosolic fractions. Student 2-tailed T-tests were performed; the difference between mitochondrial and cytosolic concentration was significant (P<0.05) in the cases of Bu and hex only.