Table 2.
Oligonucleotide sequences used as primers for polymerase chain reaction
| Gene | Primer name | Primer sequence | Product Size (bp) | References |
|---|---|---|---|---|
| tet (M) |
Tet(M)-F Tet(M)-R |
5'-GTG GAC AAA GGT ACA ACG AG-3' 5'-CGG TAA AGT TCG TCA CAC AC-3' |
406 | 15 |
| tet (O) |
Tet(O)-F Tet(O)-R |
5'-AAC TTA GGC ATT CTG GCT CAC-3' 5'-TCC CAC TGT TCC ATA TCG TCA-3' |
515 | 15 |
| tet (S) |
Tet(S)-F Tet(S)-R |
5'-CAT AGA CAA GCC GTT GAC C-3' 5'-ATG TTT TTG GAA CGC CAG AG-3' |
667 | 15 |
| tet (A) |
Tet(A)-F Tet(A)-R |
5'- GTGAAACCCAACATACCCC-3' 5'-GAAGGCAAGCAGGATGTAG-3' |
888 | 16 |
| tet (B) |
Tet(B)-F Tet(B)-R |
5'-CCTTATCATGCCAGTCTTGC-3' 5'-ACTGCCGTTTTTTCGCC-3' |
774 | 16 |