Table 2.

Fragments used to synthesize the Ubp4′′a gene; Ubp4′′a1–Ubp4′′a15 are used in the assembly protocol and Ubp4′′aFor and Ubp4′′aRev for PCR.

Ubp4′′a1	T TCGCGGTGGGCCTCGAGAATCTAGGAAATTCCTGCTATATGAACTGCATTATCC
Ubp4′′a2	CAGCTCATGCGTCCCTAAAATACACTGGATAATGCAGTTCATATAGCAGGAATTTCCTAG
Ubp4′′a3	AGTGTATTTTAGGGACGCATGAGCTGACGCAGATCTTCCTTGACGATTCGTATGCGAA
Ubp4′′a4	CCCTAGTTTAGAATTGATATTGATGTGCTTCGCATACGAATCGTCAAGGAAGA
Ubp4′′a5	GCACATCAATATCAATTCTAAACTAGGGTCGAAAGGTATTTTAGCTAAATATTTCGCTCGTCTTGTAC
Ubp4′′a6	TTGGAGCCATCTACCTGTTCCTTATACATCATATGTACAAGACGAGCGAAATATTTAGCT
Ubp4′′a7	ATATGATGTATAAGGAACAGGTAGATGGCTCCAAAAAAATTTCGATTAGCCCAATCAAATTTAAGCT
Ubp4′′a8	AACAGTGAGTTGACGGAACCACAGGCGAGCTTAAATTTGATTGGGCTAATCG
Ubp4′′a9	CGCCTGTGGTTCCGTCAACTCACTGTTTAAAACAGCTTCACAGCAAGATTGTCAGGAATTTTGCC
Ubp4′′a10	GATCTTCATGCAAACCGTCAAGCAAGAATTGGCAAAATTCCTGACAATCTTGCTGTGA
Ubp4′′a11	AATTCTTGCTTGACGGTTTGCATGAAGATCTGAACCAGTGCGGTTCTAACCCC
Ubp4′′a12	CCTCTTGGCTCAGTTCTTTCAACGGGGGGTTAGAACCGCACTGGTTCA
Ubp4′′a13	CCGTTGAAAGAACTGAGCCAAGAGGCAGAAGCGAGGCGGGAAAAGCTGAGCCTC
Ubp4′′a14	GTTCCCATTCAATACTACTGGCTATGCGGAGGCTCAGCTTTTCCCGCCTCGC
Ubp4′′a15	CGCATAGCCAGTAGTATTGAATGGGAACGCTTTCTGACCACGGATTTTAGCGTCATTGTG
Ubp4′′a16	AGCGAGAGGCATATTGCCCTTGAAAGAGGTCCACAATGACGCTAAAATCCGTGG
Ubp4′′a17	GACCTCTTTCAAGGGCAATATGCCTCTCGCTTAAAATGCAAAGTATGCAGCCACACTTCA
Ubp4′′aFor	GGGGGAATTCGATATGTTCGCGGTGGGCCTCGAGAATC
Ubp4′′bRev	GGGGGGATCCTTATGAAGTGTGGCTGCATACTTTGCATTTTAAG